Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Huotan Jiedu Tongluo Decoction Inhibits Balloon-Injury-Induced Carotid Artery Intimal Hyperplasia in the Rat through the PERK-eIF2 α -ATF4 Pathway and Autophagy Mediation

doi: 10.1155/2021/5536237

Figure Lengend Snippet: The effect of HTJDTLD on the percentage of PCNA-positive area in the CCA following BI. (a) Representative images of immunohistochemical staining for PCNA in sections of the CCA from each group (original magnification: 400×). (b) Percentage of PCNA-positive area in each group ( n = 10, mean ± SD). ∗∗ P < 0.01 vs. the Sham group. # P < 0.05, ## P < 0.01 vs. the BI group.

Article Snippet: The sections were blocked with 5% bovine serum albumin (BSA) and then incubated with an antiproliferating cell nuclear antigen (PCNA) polyclonal antibody (1 : 200; Proteintech, China) and anti-SM α -actin polyclonal antibody (1 : 200; CST, USA) overnight at 4°C followed by biotin-labeled goat anti-rabbit IgG (1 : 2000; Abcam, USA) at 37°C in the dark for 30 min. Then, diaminobenzidine (DAB; MXB, China) was used for color development, images were captured and saved with the ISCapture system, and Image-Pro Plus 6 image processing software was used for data analysis.

Techniques: Immunohistochemical staining, Staining